The circle refuses to close
Twelve fifths overshoot seven octaves.
Twelve pure fifths should land on the same pitch as seven octaves. They miss.
The miss is 23.46 cents, called the Pythagorean comma. Powers of 3 cannot equal powers of 2. A keyboard with fixed pipes or strings must place that gap somewhere. Eleven pure fifths leave a sour twelfth interval at 678 cents, called a wolf. Pietro Aron described a meantone method in 1523 CE that tuned common major thirds close to 5:4. Temperament carries the problem through seven playable steps, including a meantone wolf.
Play the pure fifth, the wolf, then the comma alone. Listen for the 3:2 fifth to settle, the 678-cent wolf to sound wide, and the close comma tones to beat. Twelve pure fifths overshoot seven octaves, so a fixed-key instrument must store or spread the 23.46-cent gap. The rough wolf makes that tuning bargain audible.